Midwest Association of Core Directors

 MWACD 2019 Abstracts

For a program overview, please click here.

Plenary Keynote

Michelle Itano, Ph.D.

Enhancing Reproducibility Through Quality Control, Date Management, and Imaging Analysis with Collaborations Across Disciplines


Currently, researchers are forced to manually track and annotate all imaging experiment steps, from sample preparation, to image data acquisition, storage, processing, and analysis, which is very onerous, slow, and wasteful. Lack of automation and poor documentation often makes it impossible to reproduce, compare, and combine large sets of data from different laboratories, biological systems, or different experimental setups, making it difficult for scientists to collaborate with each other. Our Light Microscopy Core, UNC Neuroscience Microscopy Core, has begun to address these issues through productive collaborations including other facility managers, computational scientists, individual researchers, and IT experts. One overarching collaboration is focused on how to improve the data infrastructure between researchers and Core Facilities to minimize data duplication and slow transfer rates and maximize computational resource usage for storage and analysis. This has led to multiple additional projects: to support more consistent and automatically generated image metadata and calibration control records; to implement data storage organized by research project including data generated from multiple methodologies (e.g. microscopy, MRI, mouse behavior); and to provide analysis projects that span multiple integrated methodologies (e.g. sub-second 3D mouse behavior image analysis). While still in initial stages of development, these preliminary projects suggest that Core Facility collaborations across disciplines can be harnessed to address some of the most critical issues in our field.

 Breakout Session 2 (Thursday, 1:30pm - 2:30pm)

ConCurrent Session 2A: Imaging - Serial Block Face SEM

Title: “Challenges for serial block face imaging posed by botanical material”
Dr. Christopher Gilpin, Director, Life Science Microscopy Facility, Purdue University

Title: “Practical Considerations for Starting a Serial Block Face SEM Service with Examples of Normal and Diseased Tissue Architecture”
Dr. Jeffrey Salisbury, Professor Biochemistry and Molecular Biology and Scientific Director Microscopy and Cell Analysis, Mayo Clinic Foundation

Title: “Specimen Preparation and Operation of the Serial Block Face SEM”
Trace Christensen and Adam Jaspersen, Microscopy and Cell Analysis, Mayo Clinic Foundation
Website https://www.mayo.edu/research/core-resources/microscopy-cell-analysis-core/overview

Concurrent Session 2B: Flow Cytometry - Channeling Experiments Using Multiple Technologies

Title: “Engineered Ion Channels”
Willow Coyote-Maestas, HHMI Gilliam Fellow, Department of Biochemistry, Molecular Biology & Biophysics,Bioinformatics & Computational Biology, The University of Minnesota

Title: “Flow, Sorting, IHC and Genomics- Together at Last?”
Paul E. Champoux, Director, University Flow Cytometry Resource, University of Minnesota
Website: researchservices.umn.edu/services-name/flow-cytometry-resource

 Plenary Session 2 (Friday, 8:00AM - 9:00AM)

Paul d. Robbins, Ph.D.

Medical Discovery Team on Aging - Core Integration to Understand and Extend Human Healthspan

Dr. Paul Robbins (Professor, Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota) is the Associate Director of The University of Minnesota Medical Discovery Team on Biology of Aging. Dr. Robbins is an internationally recognized expert in the molecular and cellular basis of aging whose work focuses on the role of inflammation in aging and on the discovery of drugs that may influence the aging process.

The goals of the Medical Discovery Team on Biology of Aging are to gain an understanding of the molecular events that lead to aging and develop therapeutic approaches to extend healthspan and improve the quality of human life. Dr. Robbins presentation will highlight the multiple-modal technology approaches required to meet these goals (https://cbs.umn.edu/node/14691).